RESUMEN
Cysticerci of Taenia solium cause cysticercosis, with neurocysticercosis (NCC) as the major pathology. Sensible and specific recombinant antigens would be an source of antigen for immunodiagnosis. The objective of this work was the molecular characterization and evaluation, of three news recombinant antigens (TsF78, TsP43 and TsC28), obtained by screening of a Taenia solium cDNA library. The three cDNA were analysed by bioinformatic programs, subcloned and expresed. The purified proteins were evaluated in ELISA using cyst fluid as control. TsF78 is filamina, TsP43 a peroxidase and TsC28 collagen XV. The sensitivity and specificity of the recombinant proteins were; TsF78 93.8 % and 95.0 %, TsP62 91.7 % and 93.3 %, TsC28 85.4 % and 93.3 %, respectively, while the cyst fluid showed a sensitivity of 87.5 % and a specificity of 76.7 %. Given its high sensitivity and specificity, the recombinant proteins TsF78 and TsP62 could be used in the diagnosis of cysticercosis.
Asunto(s)
Antígenos Helmínticos/inmunología , Cisticercosis/diagnóstico , Pruebas Inmunológicas , Proteínas Recombinantes/inmunología , Taenia solium/inmunología , Teniasis/diagnóstico , Animales , Antígenos Helmínticos/genética , Estudios de Casos y Controles , Cisticercosis/inmunología , Cisticercosis/microbiología , Humanos , Proteínas Recombinantes/genética , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Taenia solium/genética , Teniasis/inmunología , Teniasis/microbiologíaRESUMEN
Taenia saginata and Taenia solium, known as beef and pork tapeworm, are foodborne pathogens of global importance having a substantial impact on human health and economy. The study aimed to summarize the occurrence of human Taenia spp. infection in past 10 years in Slovakia based on reports of clinicians and diagnostic laboratories to Public Health Authority of the Slovak Republic. Altogether, 19 human cases were reported in Slovakia in 2010-2019, with the incidence of infection ranging from 0.00 to 0.12/100,000 inhabitants per year. In two patients T. saginata infection was confirmed molecularly. Nucleotide sequences of the analyzed nad1 gene fragments derived from both patients were identical and in a phylogenetic tree clustered together with T. saginata Tsa isolate (AM503345), as well as with a homologous sequence of the completely sequenced mitochondrial genome of T. saginata. Similarly, cox1 nucleotide sequences derived from one of the patients and isolates from Asia and/or Europe were identical. A cluster for cox1 partial gene sequence was placed separately from closely related Taenia asiatica and/or T. solium isolates in the phylogenetic tree.
Asunto(s)
Taenia saginata/genética , Teniasis/epidemiología , Adulto , Anciano , Animales , Niño , Preescolar , Femenino , Genes de Helminto , Humanos , Masculino , Persona de Mediana Edad , Filogenia , Eslovaquia/epidemiología , Taenia saginata/aislamiento & purificación , Teniasis/microbiología , Adulto JovenRESUMEN
La teniasis es una parasitosis intestinal causada por la forma adulta de los cestodos (gusanos planos). En el ser humano se presentan dos especies, Taenia solium y Taenia saginata, ambas hermafroditas y que requieren un huésped intermediario para cumplir su ciclo: ganado porcino y ganado bovino respectivamente. El hombre es el huésped definitivo en el cual se desarrolla la forma adulta. La clínica principal de Taenia saginata es digestiva, mientras que Taenia solium puede afectar a otros tejidos. Se presenta el caso de una adolescente que elimina parásitos en la ropa interior y heces sin otro síntoma. Los describen como gusanos blancos, planos y móviles. En la anamnesis refiere que le gustan los chuletones de carne poco cocinada, pero sin concretar un posible foco de la infestación. Se realiza un análisis con resultado normal, excepto una eosinofilia de 9,6%. En el laboratorio de Microbiología, por visión directa tras tinción con tinta china y reacción en cadena de la polimerasa directa se confirma el diagnóstico de Taenia saginata. Tras el resultado, se le indica tratamiento con prazicuantel vía oral en dosis única (medicamento off-label), sin presentar efectos secundarios (AU)
Taeniasis is an intestinal parasitic infection due to an adult worm of tapeworms (cestodes). In humans, there are two main species: Taenia solium and Taenia saginata, both of them are hermaphrodites and require an intermediate host to complete their life circle: beef tapeworm (Taenia saginata) and pork tapeworm (Taenia solium). Humans are the only definitive host in which the adult form develops. Taenia saginata manly causes gastrointestinal symptoms, while Taenia solium can affect other tissues. We report the case of a teenager who found a number of adult worms on her intimate clothes and in her stools without any other symptom. The worms were described as flat, white and mobile. At the review, she said that she loved eating undercooked meat, without determining the source of the infection. We detected high eosinophilia level in blood test (9,6%). A microbiological test identified proglottids in the stool with a direct examination, and polymerase chain reaction methods established a species diagnosis of Taenia saginata. As a result, we started treatment with prazicuantel orally single dose (off-label) without presenting any side effects (AU)
